1ZAN
Crystal structure of anti-NGF AD11 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.934 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.801, 69.354, 64.104 |
Unit cell angles | 90.00, 117.02, 90.00 |
Refinement procedure
Resolution | 17.000 - 1.700 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cic |
RMSD bond length | 0.010 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.640 | 1.750 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 47951 | |
<I/σ(I)> | 5.8 | 5.5 |
Completeness [%] | 97.2 | 78.4 |
Redundancy | 6.1 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 20% Peg4000, 600mM NaCl, 100mM BTP, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |