1Z8L
Crystal structure of prostate-specific membrane antigen, a tumor marker and peptidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.2818 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.865, 157.765, 133.839 |
Unit cell angles | 90.00, 93.24, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.500 |
Rwork | 0.252 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1de4 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.470 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.600 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 80077 | |
Completeness [%] | 99.0 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | Peg3350 and sodium thiocyanate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | Peg3350 and sodium thiocyanate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |