1Z7L
Crystal structure of fragment of mouse ubiquitin-activating enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-23 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | H 3 2 |
Unit cell lengths | 216.123, 216.123, 196.092 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.800 |
R-factor | 0.277 |
Rwork | 0.277 |
R-free | 0.30800 |
Structure solution method | MAD combine with SIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.840 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.071 | 0.230 |
Number of reflections | 43074 | |
<I/σ(I)> | 3.5 | |
Completeness [%] | 98.6 | 92.1 |
Redundancy | 5.4 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294.15 | 2mM TCEP, 1.8M ammonium sulfate, 30% glycerol, 5mM Tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294.15K |