1Z5A
Topoisomerase VI-B, ADP-bound dimer form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 74.569, 74.569, 345.557 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.19706 |
| Rwork | 0.195 |
| R-free | 0.23093 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mx0 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.196 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 52943 | |
| <I/σ(I)> | 15 | 6.8 |
| Completeness [%] | 93.1 | 84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | Tris-HCl, LiSO4, PEG-4000, glycerol, ADP, MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
