1Z57
Crystal structure of human CLK1 in complex with 10Z-Hymenialdisine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.277, 64.434, 80.112 |
| Unit cell angles | 90.00, 119.19, 90.00 |
Refinement procedure
| Resolution | 29.300 - 1.700 |
| R-factor | 0.14014 |
| Rwork | 0.138 |
| R-free | 0.18581 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1JOW.pdb |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.591 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 45196 | |
| Completeness [%] | 99.8 | 86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | PEG 6K, Bicine, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
