1Z55
Effect of alcohols on protein hydration
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2004-05-09 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.216, 79.216, 37.853 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.900 |
R-factor | 0.2181 |
Rwork | 0.218 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2lym |
RMSD bond angle | 0.004 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.100 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.021 | |
Number of reflections | 8824 | |
<I/σ(I)> | 4 | |
Completeness [%] | 88.7 | |
Redundancy | 14.1 | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | sodium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |