1Z4S
Crystal Structure of Gly19 and Glu60 deletion mutant of Human Acidic Fibroblast Growth Factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2004-11-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.466, 110.908, 68.828 |
Unit cell angles | 90.00, 103.30, 90.00 |
Refinement procedure
Resolution | 14.970 - 2.600 |
Rwork | 0.195 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jqz |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.970 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.075 | 0.314 |
Number of reflections | 21122 | |
<I/σ(I)> | 28.6 | 4.6 |
Completeness [%] | 89.8 | 100 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | Sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |