1Z4S
Crystal Structure of Gly19 and Glu60 deletion mutant of Human Acidic Fibroblast Growth Factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2004-11-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.466, 110.908, 68.828 |
| Unit cell angles | 90.00, 103.30, 90.00 |
Refinement procedure
| Resolution | 14.970 - 2.600 |
| Rwork | 0.195 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.970 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.075 | 0.314 |
| Number of reflections | 21122 | |
| <I/σ(I)> | 28.6 | 4.6 |
| Completeness [%] | 89.8 | 100 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | Sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
