1Z4Q

Structure of the D41N variant of the human mitochondrial deoxyribonucleotidase in complex with 2',3'-dideoxy-2',3-didehydrothymidine 5'-monophosphate (d4T-MP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Detector technology	CCD
Collection date	2004-01-27
Spacegroup name	P 43 21 2
Unit cell lengths	73.770, 73.770, 106.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.050
R-factor	0.17601
Rwork	0.174
R-free	0.21032
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.672
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.120
High resolution limit [Å]	2.050	2.050
Number of reflections	18971
<I/σ(I)>	21.5	4.3
Completeness [%]	99.7	100
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5		pH 4.5