1Z4M

Structure of the D41N variant of the human mitochondrial deoxyribonucleotidase in complex with uridine 5'-monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Detector technology	CCD
Collection date	2004-01-27
Spacegroup name	P 43 21 2
Unit cell lengths	73.572, 73.572, 105.748
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.700
R-factor	0.19403
Rwork	0.193
R-free	0.21527
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.631
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	32144
<I/σ(I)>	24.5	3.7
Completeness [%]	98.5	98.3
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5		pH 4.5