1Z4L

Structure of the D41N variant of the human mitochondrial deoxyribonucleotidase in complex with thymidine 5'-monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Collection date	2004-01-27
Spacegroup name	P 43 21 2
Unit cell lengths	73.646, 73.646, 106.375
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.800
R-factor	0.17438
Rwork	0.172
R-free	0.21016
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.790
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	27554
<I/σ(I)>	17.7	3.9
Completeness [%]	99.4	99.4
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5		pH 4.5