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1Z3H

The exportin Cse1 in its cargo-free, cytoplasmic state

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID14-4
Synchrotron siteESRF
BeamlineID14-4
Temperature [K]100
Detector technologyCCD
Collection date2004-03-05
DetectorADSC QUANTUM 4
Wavelength(s)0.93
Spacegroup nameP 21 21 2
Unit cell lengths162.520, 113.040, 122.970
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution122.000 - 3.100
R-factor0.287
Rwork0.241
R-free0.28800
Structure solution methodSAD
RMSD bond length0.010
RMSD bond angle1.543
Data reduction softwareMOSFLM
Data scaling softwareCCP4 ((SCALA))
Phasing softwareSHARP
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0003.480
High resolution limit [Å]3.3003.300
Number of reflections41661
<I/σ(I)>5.5
Completeness [%]98.598.5
Redundancy13.213.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8298PEG 8000, MgCl2, glycerol, DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1VAPOR DIFFUSION, HANGING DROP8298PEG 8000, MgCl2, glycerol, DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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