1Z3E
Crystal Structure of Spx in Complex with the C-terminal Domain of the RNA Polymerase Alpha Subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00003 |
| Spacegroup name | H 3 |
| Unit cell lengths | 96.440, 96.440, 57.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.22000 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.272 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.710 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.042 | 0.311 |
| Number of reflections | 31461 | |
| <I/σ(I)> | 12.5 | 2.5 |
| Completeness [%] | 99.2 | 98.5 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 273 | lithium sulfate, ammonium sulfate, sodium citrate, pH 5.6, VAPOR DIFFUSION, temperature 273K |
| 1 | VAPOR DIFFUSION | 5.6 | 273 | lithium sulfate, ammonium sulfate, sodium citrate, pH 5.6, VAPOR DIFFUSION, temperature 273K |
