1Z37

Crystal structure of Trichomonas vaginalis purine nucleoside phosphorylase complexed with adenosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Wavelength(s)	0.9766
Spacegroup name	P 41 3 2
Unit cell lengths	136.800, 136.800, 136.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.560 - 2.900
R-factor	0.208
Rwork	0.208
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pke
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.560	3.080
High resolution limit [Å]	2.900	2.900
Number of reflections	8680
Completeness [%]	85.0	78.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	PEG400, ethylene glycol, magnesium chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K