1Z2O
Inositol 1,3,4-trisphosphate 5/6-Kinase in complex with mg2+/ADP/Ins(1,3,4,6)P4
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.189, 94.294, 47.351 |
Unit cell angles | 90.00, 110.65, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.240 |
Rwork | 0.189 |
R-free | 0.20800 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.280 |
High resolution limit [Å] | 1.240 | 1.240 |
Number of reflections | 86579 | |
Completeness [%] | 98.6 | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 297 | 25% PEG 3350, 0.1 M bis-tris, 10 mM DTT, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 297 | 25% PEG 3350, 0.1 M bis-tris, 10 mM DTT, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 297 | 25% PEG 3350, 0.1 M bis-tris, 10 mM DTT, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 297 | 25% PEG 3350, 0.1 M bis-tris, 10 mM DTT, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |