1Z2M
Crystal Structure of ISG15, the Interferon-Induced Ubiquitin Cross Reactive Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-18 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 55.790, 56.940, 103.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.550 - 2.500 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.22300 |
| Structure solution method | SIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.550 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 5624 | |
| <I/σ(I)> | 10.1 | 3.7 |
| Completeness [%] | 93.9 | 88.4 |
| Redundancy | 2.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | Tris-HCl buffer, PEG4K, MgCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
