1Z26
Structure of Pyrococcus furiosus Argonaute with bound tungstate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.2139 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.100, 103.501, 73.225 |
Unit cell angles | 90.00, 102.46, 90.00 |
Refinement procedure
Resolution | 37.820 - 2.500 |
Rwork | 0.224 |
R-free | 0.28200 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 58718 | |
<I/σ(I)> | 26.4 | 3.2 |
Completeness [%] | 98.7 | 98.8 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | alcohol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 100K |