1Z1G
Crystal structure of a lambda integrase tetramer bound to a Holliday junction
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793,0.9795,0.9709 |
| Spacegroup name | P 31 |
| Unit cell lengths | 109.759, 109.759, 265.971 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 4.400 |
| R-factor | 0.24683 |
| Rwork | 0.244 |
| R-free | 0.29207 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.831 |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 4.560 |
| High resolution limit [Å] | 4.400 | 4.400 |
| Number of reflections | 22783 | |
| Completeness [%] | 99.7 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 8000, bis-tris-propane, di-ammonium hydrogen phosphate, glycerol, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | bis-tris-propane | ||
| 3 | 1 | 1 | di-ammonium hydrogen phosphate | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 1 | DTT | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | PEG 8000 | ||
| 8 | 1 | 2 | bis-tris-propane | ||
| 9 | 1 | 2 | di-ammonium hydrogen phosphate |
