1YYA
Crystal structure of TT0473, putative Triosephosphate Isomerase from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-13 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 87.612, 118.382, 120.677 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.890 - 1.600 |
R-factor | 0.174 |
Rwork | 0.174 |
R-free | 0.18200 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.054 | 0.363 |
Number of reflections | 82497 | |
<I/σ(I)> | 12.7 | 4.79 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.6 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.6 | 291 | PEG4000, SODIUM CITRATE, TRIS, SODIUM CHLORIDE, pH 5.6, MICROBATCH, temperature 291K |