1YXL
Crystal structure of a novel phospholipase A2 from Naja naja sagittifera at 1.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | CU K-ALPHA |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 42.017, 42.017, 64.451 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.477 |
| R-factor | 0.18971 |
| Rwork | 0.188 |
| R-free | 0.22961 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1psh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.798 |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.990 | 1.530 |
| High resolution limit [Å] | 1.477 | 1.477 |
| Number of reflections | 17781 | |
| <I/σ(I)> | 26.6 | 2 |
| Completeness [%] | 97.5 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1mM CaCl2, 35% ethanol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
