1YVU

Crystal structure of A. aeolicus Argonaute

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-07-22
Detector	MARRESEARCH
Wavelength(s)	0.9788, 0.9790, 0.95
Spacegroup name	P 21 21 21
Unit cell lengths	64.150, 100.679, 115.217
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.900 - 2.900
R-factor	0.208
Rwork	0.208
R-free	0.29800
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	2.000
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.900	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.111	0.472
Number of reflections	15291
Completeness [%]	99.6	98.6
Redundancy	7.6	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	PEG 550MME, calcium chloride, cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	PEG 550MME, calcium chloride, cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	PEG 550MME, calcium chloride, cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K