1YTF
YEAST TFIIA/TBP/DNA COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Detector technology | IMAGE PLATE |
Collection date | 1995-08-11 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.150, 93.050, 117.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.500 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.29700 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.290 |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 * |
High resolution limit [Å] | 2.500 * |
Rmerge | 0.050 |
Total number of observations | 196513 * |
Number of reflections | 22476 |
Completeness [%] | 97.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | complex | 5 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 25 (mM) | |
3 | 1 | reservoir | 100 (mM) | ||
4 | 1 | reservoir | 10 (mM) | ||
5 | 1 | reservoir | dithiothreitol | 1 (mM) | |
6 | 1 | reservoir | PEG6000 | 8-10 (%) |