1YT5
Crystal structure of NAD kinase from Thermotoga maritima
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 131.448, 137.153, 58.253 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.300 |
R-factor | 0.217 |
Rwork | 0.213 |
R-free | 0.28000 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.539 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.000 | 2.358 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.091 | 0.330 |
Number of reflections | 44304 | |
<I/σ(I)> | 15 | 3.2 |
Completeness [%] | 90.1 | 87 |
Redundancy | 4.8 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | Ammonium Sulfate, pH 6.5, VAPOR DIFFUSION, temperature 298.0K |