1YS4
Structure of Aspartate-Semialdehyde Dehydrogenase from Methanococcus jannaschii
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9743, 0.9797, 0.9796 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 95.254, 95.254, 297.957 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.220 - 2.290 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.22100 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.330 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 61981 | |
Completeness [%] | 99.1 | 98.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 298 | sodium malonate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.00 |