1YRJ
Crystal Structure of Apramycin bound to a Ribosomal RNA A site oligonucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.127 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.250, 30.900, 45.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.700 |
Rwork | 0.246 |
R-free | 0.30700 |
Structure solution method | MIR |
Starting model (for MR) | 1lc4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.630 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 | 2.790 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.073 | 0.170 |
Number of reflections | 3802 | |
<I/σ(I)> | 43 | |
Completeness [%] | 99.0 | 99 |
Redundancy | 5.5 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 310 | MPD, sodium cacodylate, NaCl, KCl, MgSO4, pH 6.5, EVAPORATION, temperature 310K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | sodium cacodylate | ||
3 | 1 | 1 | NaCl | ||
4 | 1 | 1 | KCl | ||
5 | 1 | 1 | MgSO4 | ||
6 | 1 | 1 | H2O |