1YQU
Escherichia coli purine nucleoside phosphorylase II, the product of the xapA gene
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Detector technology | CCD |
Collection date | 2002-03-04 |
Detector | MARRESEARCH |
Wavelength(s) | 0.811 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 71.345, 71.345, 267.537 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 3.100 |
R-factor | 0.2461 |
Rwork | 0.245 |
R-free | 0.26740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pnp |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.126 | 0.382 |
Number of reflections | 15055 | |
<I/σ(I)> | 11 | 2.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 294 | Tris, PEG4K, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 294K |