1YQQ
Escherichia coli purine nucleoside phosphorylase II, the product of the xapA gene
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Detector technology | IMAGE PLATE |
Collection date | 2002-05-08 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 98.531, 116.633, 73.423 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.2408 |
Rwork | 0.240 |
R-free | 0.25840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | trignonal crystal form of PNP-II |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.070 | 0.328 |
Number of reflections | 26677 | |
<I/σ(I)> | 20 | 3.3 |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 6.8 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 294 | glycine, PEG4K, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |