1YQ2
beta-galactosidase from Arthrobacter sp. C2-2 (isoenzyme C2-2-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9168 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 140.091, 205.698, 140.458 |
| Unit cell angles | 90.00, 102.34, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.15702 |
| Rwork | 0.157 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dp0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.472 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 577572 | |
| <I/σ(I)> | 10.3 | 2.1 |
| Completeness [%] | 95.0 | 81 |
| Redundancy | 2.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | PEG 4000, sodium chloride, amonium sulphate, sodium citrate buffer, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
