1YOM
Crystal structure of Src kinase domain in complex with Purvalanol A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.698, 63.078, 74.055 |
| Unit cell angles | 100.61, 88.86, 89.89 |
Refinement procedure
| Resolution | 12.000 - 2.900 |
| R-factor | 0.22187 |
| Rwork | 0.217 |
| R-free | 0.31322 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.692 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.800 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| <I/σ(I)> | 4.6 | 1.4 |
| Completeness [%] | 93.0 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 20% ethylene glycol , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
