1YO8
Structure of the C-terminal domain of human thrombospondin-2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2662,1.0213,1.0452,1.0210 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 93.437, 121.588, 155.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.224 |
| Rwork | 0.218 |
| R-free | 0.28400 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.154 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 49844 | |
| <I/σ(I)> | 18.5 | 2.7 |
| Completeness [%] | 93.8 | 60.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | Tris-HCl, Sodium Acetate, PEG 4000, glycine, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 8.50 |
