1YO5
Analysis of the 2.0A crystal structure of the protein-DNA complex of human PDEF Ets domain bound to the prostate specific antigen regulatory site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2004-08-09 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.160, 71.476, 39.136 |
Unit cell angles | 90.00, 113.45, 90.00 |
Refinement procedure
Resolution | 35.900 - 2.000 |
R-factor | 0.2049 |
Rwork | 0.203 |
R-free | 0.23889 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dux |
RMSD bond length | 0.009 |
RMSD bond angle | 1.314 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.900 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.082 | 0.230 |
Number of reflections | 11176 | |
<I/σ(I)> | 17.1 | 6.6 |
Completeness [%] | 94.7 | 85.4 |
Redundancy | 3.74 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | PEG 4000, Citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | Citrate | ||
3 | 1 | 2 | PEG 4000 | ||
4 | 1 | 2 | Citrate, |