1YO5
Analysis of the 2.0A crystal structure of the protein-DNA complex of human PDEF Ets domain bound to the prostate specific antigen regulatory site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.160, 71.476, 39.136 |
| Unit cell angles | 90.00, 113.45, 90.00 |
Refinement procedure
| Resolution | 35.900 - 2.000 |
| R-factor | 0.2049 |
| Rwork | 0.203 |
| R-free | 0.23889 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dux |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.314 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.900 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.082 | 0.230 |
| Number of reflections | 11176 | |
| <I/σ(I)> | 17.1 | 6.6 |
| Completeness [%] | 94.7 | 85.4 |
| Redundancy | 3.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | PEG 4000, Citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | Citrate | ||
| 3 | 1 | 2 | PEG 4000 | ||
| 4 | 1 | 2 | Citrate, |
