1YLA
Ubiquitin-conjugating enzyme E2-25 kDa (Huntington interacting protein 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-10 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.831, 55.022, 61.762 |
| Unit cell angles | 64.19, 74.61, 69.89 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tte |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.054 | 0.164 |
| Number of reflections | 15647 | |
| <I/σ(I)> | 11 | 3 |
| Completeness [%] | 96.1 | 96.1 |
| Redundancy | 2.23 | 2.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | PEG 8000, calcium acetate, cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K, pH 6.50 |
