1YL5
Crystal structure of Mycobacterium tuberculosis dihydrodipicolinate reductase (RV2773C) (crystal form A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-05-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.10 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 59.260, 122.000, 77.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.20697 |
Rwork | 0.205 |
R-free | 0.24924 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p9l |
RMSD bond length | 0.013 |
RMSD bond angle | 1.529 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.085 | 0.733 |
Number of reflections | 25757 | |
<I/σ(I)> | 17.1 | 1.75 |
Completeness [%] | 99.6 | 99.1 |
Redundancy | 63.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 100 mM Tris/HCl, 20 % PEG 3350, 120 mM MgCl2 , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |