1YKF
NADP-DEPENDENT ALCOHOL DEHYDROGENASE FROM THERMOANAEROBIUM BROCKII
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1995-04-27 |
| Detector | WEISSENBERG |
| Spacegroup name | P 65 |
| Unit cell lengths | 80.614, 80.614, 400.335 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.26700 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 23.800 * |
| Data reduction software | HKL ((DENZO)) |
| Data scaling software | HKL ((SCALEPACK)) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.105 | 0.254 |
| Number of reflections | 44669 | 2547 * |
| <I/σ(I)> | 16.3 | 4.3 |
| Completeness [%] | 88.4 | 75.8 |
| Redundancy | 2.4 | 1.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.3 * | 293 * | pH 8.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.8 (mg/ml) | |
| 10 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 2 | 1 | drop | NADP+ | 2 (mM) | |
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | dithiothreitol | 0.1 (mM) | |
| 6 | 1 | drop | 0.05 (mM) | ||
| 7 | 1 | drop | PEG4000 | 14 (%) | |
| 8 | 1 | reservoir | PEG4000 | 15.5 (%) | |
| 9 | 1 | reservoir | 50 (mM) |
