1YJS

K226Q Mutant Of Serine Hydroxymethyltransferase From B. Stearothermophilus, Complex With Glycine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-03-11
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	61.365, 106.287, 56.981
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.20344
Rwork	0.203
R-free	0.22701
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kkj
RMSD bond length	0.011
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge		0.149
Number of reflections	25927
<I/σ(I)>	20.7
Completeness [%]	97.5	98.7
Redundancy	2.4	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	Hepes, MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K