1YIX
Crystal structure of YCFH, TATD homolog from Escherichia coli K12, at 1.9 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.387, 76.322, 80.738 |
| Unit cell angles | 90.00, 92.30, 90.00 |
Refinement procedure
| Resolution | 19.870 - 1.900 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j6o |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 0.231 |
| Number of reflections | 39039 | |
| <I/σ(I)> | 18.6 | 3.5 |
| Completeness [%] | 93.4 | 67 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2 M sodium acetate trihydrate, 0.1M Tris hydrochloride, pH 8.5, 30% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
