1YI7
Beta-d-xylosidase (selenomethionine) XYND from Clostridium Acetobutylicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.130, 143.730, 188.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.144 |
| Rwork | 0.142 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y7b |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.379 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 0.380 |
| Number of reflections | 190582 | |
| <I/σ(I)> | 10.8 | 4.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.8 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1 M HEPES, 0.2 M AMMONIUM SULFATE, 15% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
