1YI5

Crystal structure of the a-cobratoxin-AChBP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-14
Detector	ADSC QUANTUM 4
Wavelength(s)	0.975
Spacegroup name	C 2 2 21
Unit cell lengths	162.604, 313.415, 106.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 4.200
R-factor	0.33339
Rwork	0.331
R-free	0.37796
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1UX2 2ctx
RMSD bond length	0.012
RMSD bond angle	1.405
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	4.200
Rmerge	0.160
Number of reflections	19232
<I/σ(I)>	4.5
Completeness [%]	95.1
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.9M NA-CITRATE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K