1YHU

Crystal structure of Riftia pachyptila C1 hemoglobin reveals novel assembly of 24 subunits.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	P 41 21 2
Unit cell lengths	195.840, 195.840, 308.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.150
R-factor	0.2433
Rwork	0.243
R-free	0.27070
Structure solution method	Plus molecular averaging
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.150	3.150
Number of reflections	104444
<I/σ(I)>	11.3	2.4
Completeness [%]	100.0	99.9
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	1.6M ammonium sulfate, 0.1M HEPES, 0.1M NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K