1YHN
Structure basis of RILP recruitment by Rab7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-29 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9175 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 93.134, 93.134, 132.826 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
Rwork | 0.268 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t91 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.682 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.097 | 0.426 |
Number of reflections | 7272 | |
<I/σ(I)> | 6.6 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.3 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | ammonium sulfate, polyvinylpyrrolidone K15, HEPS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |