1YFR
crystal structure of alanyl-tRNA synthetase in complex with ATP and magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2004-07-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 173.602, 73.926, 73.976 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.150 |
R-factor | 0.2 |
Rwork | 0.194 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1riq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.056 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.035 | 0.250 |
Number of reflections | 50235 | |
<I/σ(I)> | 20.4 | |
Completeness [%] | 94.8 | 96.9 |
Redundancy | 4.7 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.2 | 298 | PEG 5000 monomethyl ether, NaCl, Tris-Cl, Tris-base, ATP, MgCl2, pH 6.2, VAPOR DIFFUSION, temperature 298K |