1YDY
Crystal structure of periplasmic glycerophosphodiester phosphodiesterase from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-10-12 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.789, 74.469, 157.219 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.940 - 1.700 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t8q |
RMSD bond length | 0.013 |
RMSD bond angle | 1.600 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.083 | 0.530 |
Number of reflections | 82266 | |
<I/σ(I)> | 18.1 | 1.8 |
Completeness [%] | 98.8 | 93.9 |
Redundancy | 4 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2 M sodium chloride, 0.1 M Tris, 25% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |