1YDN
Crystal Structure of the HMG-CoA Lyase from Brucella melitensis, Northeast Structural Genomics Target LR35.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9878 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.272, 86.399, 87.683 |
Unit cell angles | 90.00, 118.70, 90.00 |
Refinement procedure
Resolution | 28.940 - 2.300 |
R-factor | 0.271 |
Rwork | 0.271 |
R-free | 0.30400 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.940 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.100 | 0.243 |
Number of reflections | 97811 | |
<I/σ(I)> | 13.91 | 0.219 |
Completeness [%] | 99.6 | 99.3 |
Redundancy | 3.97 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20% PEG3350, 200 mM CaCl2, and 10 mM DTT., pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |