1YC0
short form HGFA with first Kunitz domain from HAI-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 76.218, 76.218, 176.236 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.850 - 2.600 |
| R-factor | 0.19611 |
| Rwork | 0.195 |
| R-free | 0.21811 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fak |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.096 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.07) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.078 | 0.407 |
| Number of reflections | 17975 | |
| <I/σ(I)> | 17 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 50% MPD, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
