1YBO
Crystal structure of the PDZ tandem of human syntenin with syndecan peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 72.322, 72.322, 125.921 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.300 |
| Rwork | 0.193 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n99 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.613 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.283 |
| Number of reflections | 23083 | |
| <I/σ(I)> | 30.3 | 8.31 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 8.3 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 20% PEG 3350, 0.2M NH4Cl, VAPOR DIFFUSION, SITTING DROP |
