1YA5
Crystal structure of the titin domains z1z2 in complex with telethonin
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-06 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.326, 63.213, 242.584 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.920 - 2.445 |
R-factor | 0.23278 |
Rwork | 0.232 |
R-free | 0.26496 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.370 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.920 | 2.490 |
High resolution limit [Å] | 2.445 | 2.445 |
Rmerge | 0.053 | 0.395 |
Number of reflections | 25248 | |
<I/σ(I)> | 18.2 | 2.1 |
Completeness [%] | 86.9 | |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.45 | 298 | pH 4.45, VAPOR DIFFUSION, HANGING DROP, temperature 298K |