1Y8Z

alpha-glucosyltransferase in complex with UDP and a 13-mer DNA containing a HMU base at 1.9 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-23
Detector	MARRESEARCH
Wavelength(s)	0.934
Spacegroup name	P 1 21 1
Unit cell lengths	41.263, 114.655, 86.143
Unit cell angles	90.00, 89.49, 90.00

Refinement procedure

Resolution	20.000 - 1.900
Rwork	0.208
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y6f
RMSD bond length	0.005
RMSD bond angle	1.212
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	62256
<I/σ(I)>	7.8	2.2
Completeness [%]	99.0	93.6
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	PEG10000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG10000
2	1	1	H2O
3	1	2	PEG10000
4	1	2	H2O