1Y8N
Crystal structure of the PDK3-L2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-03 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 120.806, 120.806, 238.587 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.690 - 2.600 |
| R-factor | 0.21147 |
| Rwork | 0.210 |
| R-free | 0.24795 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDK2 PDB entry 1JM6 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.690 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.053 | 0.545 |
| Number of reflections | 32450 | |
| <I/σ(I)> | 28.7 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.7 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | soudium citrate, sodium potassium phosphate, sodium chrolide, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
