1Y6R
Crystal structure of MTA/AdoHcy nucleosidase complexed with MT-ImmA.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-04-06 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.51418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.570, 69.600, 127.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.800 - 2.200 |
Rwork | 0.193 |
R-free | 0.24400 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | : 1NC1 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | d*TREK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.810 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 27476 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | PEG 200, sodium acetate, sodium chloride, cobaltous chloride hexahydrate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |