1Y6Q

Cyrstal structure of MTA/AdoHcy nucleosidase complexed with MT-DADMe-ImmA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-11-04
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	51.710, 69.790, 128.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.220 - 2.200
Rwork	0.198
R-free	0.24600
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1nc1
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	d*TREK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.220	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	24071

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	sodium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K