1Y63

Initial crystal structural analysis of a probable kinase from Leishmania major Friedlin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-06
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9198, 0.8856, 0.9202
Spacegroup name	P 4 21 2
Unit cell lengths	107.116, 107.116, 35.216
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.000 - 1.700
R-factor	0.1665
Rwork	0.165
R-free	0.19621
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.467
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	1.630
High resolution limit [Å]	1.550	1.550
Number of reflections	30411
<I/σ(I)>	14.5	2
Completeness [%]	99.2	94.8
Redundancy	6	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	PEG20000, MES, MnCl2, HEPES, NaCl, Glycerol, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K