1Y63
Initial crystal structural analysis of a probable kinase from Leishmania major Friedlin
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-06 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9198, 0.8856, 0.9202 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 107.116, 107.116, 35.216 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.000 - 1.700 |
R-factor | 0.1665 |
Rwork | 0.165 |
R-free | 0.19621 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.467 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.000 | 1.630 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 30411 | |
<I/σ(I)> | 14.5 | 2 |
Completeness [%] | 99.2 | 94.8 |
Redundancy | 6 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | PEG20000, MES, MnCl2, HEPES, NaCl, Glycerol, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |